Chronic ethanol treatment induced hepatic injury, inflammation in the liver and elevation of serum glucose and triglycerides. Adult zebrafish were exposed to 0.2% ethanol for 4 weeks. (A) Hematoxylin and eosin (H&E) staining and Picrosirius Red staining in the liver of untreated and 0.2% ethanol treated adult zebrafish (top and middle). Control livers showed normal hepatocytes (n = 8/10) and ethanol treated siblings showed hepatic injury (n = 9/10). Control livers showed thin layer of picrosirius red positive staining surrounding blood vessels (n = 6/6), ethanol exposed group showed intense staining of fibrotic tissue in some cases (n = 2/5) Scale bar = 50 μm (top, bottom), 25 μm (middle). (B) Alanine aminotransferase assay from blood samples of control, 0.2% ethanol exposed zebrafish. (C) Relative mRNA expression of genes associated with inflammation and liver injury. RNAs extracted from three to five siblings were used for analysis. (D) Quantification of serum glucose in control and ethanol treated zebrafish. (E) Quantification of serum triglycerides in control and ethanol treated zebrafish. Error bars indicate standard deviation of the mean. * p < 0.05, ** p < 0.005.

Chronic ethanol treatment induced steatosis in the liver of adult zebrafish. (A) The relative mRNA expression of genes associated with lipid metabolism include lipogenic transcription factors, lipid synthesis, lipid uptake, and fatty acid transporting into the mitochondria. (B) Oil red O staining the section of liver in untreated control (left) and 0.2% ethanol treated wild type zebrafish. Scale bar = 50 μm. (C) The quantification of triglycerides in the liver of control and ethanol treated zebrafish. Error bars indicate standard deviation of the mean. * p < 0.05, ** p < 0.005.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.