Chronic ethanol treatment induced hepatic injury, inflammation in the liver and elevation of serum glucose and triglycerides. Adult zebrafish were exposed to 0.2% ethanol for 4 weeks. (A) Hematoxylin and eosin (H&E) staining and Picrosirius Red staining in the liver of untreated and 0.2% ethanol treated adult zebrafish (top and middle). Control livers showed normal hepatocytes (n = 8/10) and ethanol treated siblings showed hepatic injury (n = 9/10). Control livers showed thin layer of picrosirius red positive staining surrounding blood vessels (n = 6/6), ethanol exposed group showed intense staining of fibrotic tissue in some cases (n = 2/5) Scale bar = 50 μm (top, bottom), 25 μm (middle). (B) Alanine aminotransferase assay from blood samples of control, 0.2% ethanol exposed zebrafish. (C) Relative mRNA expression of genes associated with inflammation and liver injury. RNAs extracted from three to five siblings were used for analysis. (D) Quantification of serum glucose in control and ethanol treated zebrafish. (E) Quantification of serum triglycerides in control and ethanol treated zebrafish. Error bars indicate standard deviation of the mean. * p < 0.05, ** p < 0.005.
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