(A) Schematic representation of the constructs used for the generation of the enhancer trap line Et(kita:GalTA4,UAS:mCherry)hzm1 and Tg(UAS:eGFP-HRAS_G12V)io6 line. (B) A 5-month-old transgenic fish displaying a large black tumor in the stalk of the caudal fin. (C) Quantification of the number of fish displaying a black fin (BF) phenotype and tumor (TU) at 4 months (mean + standard deviation, n = 150).

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

(A, C, E) Confocal images of single scans through sections stained for different histone marks (as indicated on top right corner) in magenta. Inset in (A) shows the same section imaged with Normaski optics for orientation. M, melanoma tissue fluorescing in green because it expresses eGFP:HRAS_G12V; S, normal skin. (B, D, F) Close-up image showing the nuclei of melanoma cells, immunostained for the marks indicated on the left panel and counterstained with DAPI. Scale bars = 20μm.

(A) Confocal image of single scans through sections of a wild-type control fin stained for H3K27me3 in magenta. (B–F) Confocal images of single scans through sections with tumors stained for different histone marks (as indicated on top right corner) in magenta. Inset in (C) shows the same section imaged with Normaski optics for orientation. M, melanoma tissue fluorescing in green because it expresses eGFP:HRAS_G12V; S, normal skin. Scale bars = 20μm.