FIGURE

Fig. 1

ID

ZDB-FIG-210520-52

Publication

Mitchell et al., 2021 - The alx3 gene shapes the zebrafish neurocranium by regulating frontonasal neural crest cell differentiation timing

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Fig. 1

(A) Post-migratory NCCs are double transgenic (fli1a:EGFP;sox10:mRFP) and reside in the frontonasal region and in the anterior and posterior pharyngeal arches. Double transgenic animals were dissociated into single-cell suspension. (B) Scatter plot from FACS. Dashed box indicates double-positive cells sorted for single-cell transcriptomics. (C) scRNA-seq clusters double-transgenic NCCs into four distinct populations. (D-F) Genes enriched in each cluster indicate population identity. Example plots for the genes shown in bold are displayed. All individual feature plots have the same scale indicated in D.

Expression Data

Genes:	actc1b alx3 alx4a anxa2a barx1 cldn11a cryba2b cyp26a1 cyp26b1 dhrs3b dlx3b dlx4b dlx6a efnb2a entpd1 fgf20b hand2 hapln1a hoxa2b hoxa3a hoxa9a hoxb1b hoxb2a hoxb3a hoxc1a hoxd4a irx5a mab21l1 meis2b nr2f1b pitx2 pmp22b prdm1a rhoub rspo3 wfdc2 wnt11 zic2a
Fish:	vu234Tg; y1Tg
Anatomical Terms:	chondrocranium cartilage neural crest cell pharyngeal arch 1 neural crest cell pharyngeal arch 2 neural crest cell pharyngeal arch 3-7 neural crest cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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