FIGURE

Fig. 3

ID

ZDB-FIG-240916-119

Publication

Williams et al., 2024 - Keratin 8/18a.1 Expression Influences Embryonic Neural Crest Cell Dynamics and Contributes to Postnatal Corneal Regeneration in Zebrafish

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Fig. 3

Krt8/Krt18a.1 knockdown disrupts ocular and craniofacial development. Wholemount imaging showed that compared with their uninjected (A) and standard control (std ctrl) MO-injected (B) counterparts, krt8 MO-injected zebrafish embryos (C) developed microphthalmic eyes (asterisk) with coloboma (arrowhead), while krt18a.1 MO-injected embryos (D) had severely underdeveloped or anophthalmic eyes (asterisk) at 48 hpf, suggesting that the effects were more severe with Krt18a.1 knockdown. The absence of pharyngeal arch (orange arrows) and jaw (red arrows) formation was observed after MO knockdown in both cases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; mpv17^a9/a9 + MO2-krt8 mitfa^w2/w2; mpv17^a9/a9 + MO2-krt18a.1
Knockdown Reagents:	MO2-krt8 MO2-krt18a.1
Observed In:	eye optic furrow pharyngeal arch ventral mandibular arch
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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