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Fig. 3 Krt8/Krt18a.1 knockdown disrupts ocular and craniofacial development. Wholemount imaging showed that compared with their uninjected (A) and standard control (std ctrl) MO-injected (B) counterparts, krt8 MO-injected zebrafish embryos (C) developed microphthalmic eyes (asterisk) with coloboma (arrowhead), while krt18a.1 MO-injected embryos (D) had severely underdeveloped or anophthalmic eyes (asterisk) at 48 hpf, suggesting that the effects were more severe with Krt18a.1 knockdown. The absence of pharyngeal arch (orange arrows) and jaw (red arrows) formation was observed after MO knockdown in both cases.