FIGURE

Fig. 6

ID

ZDB-FIG-240916-122

Publication

Williams et al., 2024 - Keratin 8/18a.1 Expression Influences Embryonic Neural Crest Cell Dynamics and Contributes to Postnatal Corneal Regeneration in Zebrafish

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Fig. 6

Changes in retinoic acid levels affect early ocular and craniofacial development. Wholemount in situ hybridization was performed using a chromogenic (blue) colorimetric assay. Lateral images (A–D,E–H) of treated zebrafish embryos taken at 48 hpf show the teratogenic effects of pharmacological insult with 10 μM DEAB and 100 nM ATRA on the ocular and craniofacial neural crest during early development. The solid and dashed circles highlight the reduced eye size. The red and orange arrows highlight jaw and pharyngeal arch malformation, respectively. (I) Quantitative analysis of this effect shows that treatment with both DEAB and ATRA delayed ocular development and significantly decreased the eye size of the treated fish, as measured along the anterior–posterior (a-p) and dorsal-ventral (d-v) axes. *, p-value < 0.05; n.s., not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; mpv17^a9/a9
Conditions:	chemical treatment by environment: all-trans-retinoic acid chemical treatment by environment: 4-(diethylamino)benzaldehyde
Observed In:	eye adaxial-abaxial axis eye anterior-posterior axis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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