Transgenic Construct

Tg(5xUAS-E1B:6xMYC-notch1a)

ID

ZDB-TGCONSTRCT-070117-24

Previous Names

NICD (1)
Tg(5xUAS-E1b:6xMYC-notch1a)
Tg(UAS:myc-Notch1a-intra)
UAS-myc-Notch1a:intra (1)

Type

engineered_plasmid

Regulatory Regions

E1B
UAS (1)

Coding Sequences

notch1a (1)

Contains Sequences

MYC (1)

Note

This construct utilizes five copies of an optimized UAS from yeast and the minimal E1b promoter to drive the expression of a protein in which six myc epitopes are fused to the intracellular domain of the zebrafish notch1a protein.

Plasmid Map

None

Genomic Features

That Utilize Tg(5xUAS-E1B:6xMYC-notch1a) Construct

Genomic Feature	Affected Genomic Regions
kca3Tg

Transgenics

That Utilize Tg(5xUAS-E1B:6xMYC-notch1a) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
bw9Tg; kca3Tg			2 figures from 2 publications
bw9Tg; kca3Tg + MO2-tnfrsf1b		Fig. 3 from Espín-Palazón et al., 2014	Fig. 3 from Espín-Palazón et al., 2014
bw9Tg; kca3Tg + MO4-tnfa		Fig. 3 from Espín-Palazón et al., 2014	Fig. 3 from Espín-Palazón et al., 2014
bw9Tg; kca3Tg + MO6-notch3		Fig. 5 from Kim et al., 2014	Fig. 5 from Kim et al., 2014
cj3Tg; kca3Tg			2 figures from Collins et al., 2010
dctn1a^s309/s309; kca3Tg; kca4Tg (TL)	dctn1a	Fig. 6 from Del Bene et al., 2008
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	f11r.1	Figure 8 from Kondo et al., 2023	Figure 8 from Kondo et al., 2023
f11r.1^sd43/sd43; kca3Tg; ubs4Tg	f11r.1
gt1Tg; jh11Tg; kca3Tg; kca4Tg		Fig. S12 from Huang et al., 2014	Fig. S12 from Huang et al., 2014
hdac1^{hi1618Tg/hi1618Tg}; kca3Tg; kca4Tg	hdac1	Fig. 5 from Burns et al., 2009	Fig. 5 from Burns et al., 2009
hu10049Tg; kca3Tg
hu10049Tg; kca3Tg + MO1-gata1a			Fig. 6 from Chen et al., 2017
hu10049Tg; kca3Tg + MO1-tnnt2a		Fig. 4 from Chen et al., 2017
hu4008Tg; kca3Tg; kca4Tg; zf131Tg			Fig. 6 from Grotek et al., 2013
hzm12Et/hzm12Et; kca3Tg		Fig. 2 from Troll et al., 2018
hzm7Et; kca3Tg			Fig. 5 from Kim et al., 2014
hzm7Et; kca3Tg + CRISPR3-jag2b + CRISPR4-jag2b + CRISPR5-jag2b + CRISPR6-jag2b		Fig. 3 from Wada et al., 2022	Fig. 3 from Wada et al., 2022
hzm7Et; kca3Tg + MO6-notch3		Fig. 5 from Kim et al., 2014	Fig. 5 from Kim et al., 2014
ia5Tg; kca3Tg; mu286Tg; s940Tg		Figure 4 from Paolini et al., 2021	Figure 4 from Paolini et al., 2021
kca3Tg			5 figures from 4 publications
kca3Tg/+
kca3Tg/+ (AB)
kca3Tg/+; kca4Tg/+		9 figures from 6 publications	14 figures from 9 publications
kca3Tg/+; kca4Tg/+ + MO1-atoh1b		Fig. 6 from Millimaki et al., 2007	Fig. 6 from Millimaki et al., 2007
kca3Tg/+; kca4Tg/+ + MO1-wnt16 + MO2-wnt16			Fig. 4 from Clements et al., 2011
kca3Tg/+; kca4Tg/+; knu2Tg/+
kca3Tg/+; kca4Tg/+; s939Tg; um14Tg			Fig. 6 from Delous et al., 2012
kca3Tg/+; kca4Tg/+; sb1Tg/+			Fig. 5 from Kishimoto et al., 2013
kca3Tg/+; kca4Tg/+; y83Tg/+
kca3Tg/+; m1080Tg/+			Fig. 8 from Zecchin et al., 2007
kca3Tg/+; m1080Tg/m1080Tg			Fig. 8 from Zecchin et al., 2007
kca3Tg/+; vu12Tg; vu22Tg		Fig. 5 from Shin et al., 2007
kca3Tg/kca3Tg; kca4Tg/kca4Tg		Fig. 7 from Kapsimali et al., 2011	2 figures from 2 publications
kca3Tg; kca4Tg		14 figures from 12 publications	31 figures from 25 publications
kca3Tg; kca4Tg + MO1-angptl1a + MO2-angptl2b		Fig. 2 from Lin et al., 2015	Fig. 2 from Lin et al., 2015
kca3Tg; kca4Tg + MO1-arl4aa			Fig. 6 from Guo et al., 2020
kca3Tg; kca4Tg + MO1-epas1a + MO3-epas1b			Fig. 6 from Gerri et al., 2018
kca3Tg; kca4Tg + MO1-f11r.1			Fig. S7 from Kobayashi et al., 2014
kca3Tg; kca4Tg + MO1-hif1aa + MO1-hif1ab			Fig. 6 from Gerri et al., 2018
kca3Tg; kca4Tg + MO1-itgb1b			Fig. 6 from Rho et al., 2019
kca3Tg; kca4Tg + MO1-jam2a			Fig. S7 from Kobayashi et al., 2014
kca3Tg; kca4Tg + MO1-tbx2		Fig. 9 from Drummond et al., 2017	Fig. 9 from Drummond et al., 2017
kca3Tg; kca4Tg + MO2-brca2		Fig. 11 from Kroeger et al., 2017	Fig. 11 from Kroeger et al., 2017
kca3Tg; kca4Tg + MO2-gmds		Fig. 6 from Song et al., 2010
kca3Tg; kca4Tg + MO2-tnfrsf1b		Fig. 3 from Espín-Palazón et al., 2014	Fig. 3 from Espín-Palazón et al., 2014
kca3Tg; kca4Tg + MO3-mecom + MO4-mecom		Fig. 7 from Li et al., 2014	Fig. 7 from Li et al., 2014
kca3Tg; kca4Tg + MO3-plcg1		Fig. 4 from Burns et al., 2009	Fig. 4 from Burns et al., 2009
kca3Tg; kca4Tg + MO4-rap1b			Fig. 3 from Rho et al., 2019
kca3Tg; kca4Tg + MO4-tbx16		Fig. 4 from Burns et al., 2009	Fig. 4 from Burns et al., 2009
kca3Tg; kca4Tg + MO4-tnfa		Fig. 3 from Espín-Palazón et al., 2014	Fig. 3 from Espín-Palazón et al., 2014
kca3Tg; kca4Tg + MO5-mecom + MO6-mecom			Fig. 4 from Konantz et al., 2016
kca3Tg; kca4Tg + MO6-notch3		Fig. 3 from Kim et al., 2014	Fig. 3 from Kim et al., 2014
kca3Tg; kca4Tg; knu3Tg			Fig. 5 from Quillien et al., 2011
kca3Tg; kca4Tg; la2Tg; s939Tg		Fig. 4 from Sawamiphak et al., 2014	Fig. 4 from Sawamiphak et al., 2014
kca3Tg; kca4Tg; la2Tg; s939Tg + MO1-ifng1		Fig. 4 from Sawamiphak et al., 2014	Fig. 4 from Sawamiphak et al., 2014
kca3Tg; kca4Tg; la781Tg			Fig. 2 from Lee et al., 2009
kca3Tg; kca4Tg; nl1Tg			Fig. 5 from Mizoguchi et al., 2011
kca3Tg; kca4Tg; pd1Tg (AB)			Fig. 6 from Lee et al., 2014
kca3Tg; kca4Tg; s843Tg			Fig. 1 from Lee et al., 2009
kca3Tg; kca4Tg; um14Tg; vc6Tg			Fig. 5 from Samsa et al., 2015
kca3Tg; kca4Tg; um14Tg; vc6Tg + MO1-tnnt2a		Fig. 5 from Samsa et al., 2015	Fig. 5 from Samsa et al., 2015
kca3Tg; kca4Tg; vu12Tg			Fig. 5 from Shin et al., 2007
kca3Tg; kca4Tg; x17Tg		Fig. 8 from Kantarci et al., 2015	Fig. 8 from Kantarci et al., 2015
kca3Tg; kca4Tg; y1Tg		Fig. 1 from Siekmann et al., 2007
kca3Tg; kca4Tg; y83Tg			Fig. 4 from Grotek et al., 2013
kca3Tg; mu101Tg			Fig. 2 from Espanola et al., 2020
kca3Tg; mu101Tg + MO5-mecom + MO6-mecom			Fig. 4 from Konantz et al., 2016
kca3Tg; mu101Tg; nkuasgfp1aTg		Fig. 2 from Bussmann et al., 2011	Fig. 2 from Bussmann et al., 2011
kca3Tg; nkhspGFF53AEt; nkuasgfp1aTg		Fig. 6 from Faucherre et al., 2011	Fig. 6 from Faucherre et al., 2011
kca3Tg; nkuasrfp1aTg; ubs3Tg; zf169Tg + MO5-mecom + MO6-mecom		Fig. 5 from Konantz et al., 2016	Fig. 5 from Konantz et al., 2016
kca3Tg; nns5Tg; nns6Tg		Fig. S3 from Kimura et al., 2008	Fig. S3 from Kimura et al., 2008
kca3Tg; nns6Tg
kca3Tg; pd1023Tg		Fig. 7 from Ellis et al., 2013
kca3Tg; rk8Tg; ubs3Tg		Fig. 3 from Rochon et al., 2015	Fig. 3 from Rochon et al., 2015
kca3Tg; ru1012Tg; vo8Tg		2 figures from Jacobo et al., 2019
kca3Tg; s1001tEt; s1999tTg		Fig. 6 from Faucherre et al., 2011	Fig. 6 from Faucherre et al., 2011
kca3Tg; s1006tEt		Fig. 6 from Faucherre et al., 2011
kca3Tg; ubs3Tg			Fig. 7 from Alghisi et al., 2013
kca3Tg; ubs4Tg			2 figures from 2 publications
kca3Tg; ubs4Tg + MO1-epas1a + MO3-epas1b			Fig. 6 from Gerri et al., 2018
kca3Tg; ubs4Tg + MO1-f11r.1			Fig. S7 from Kobayashi et al., 2014
kca3Tg; ubs4Tg + MO1-hif1aa + MO1-hif1ab			Fig. 6 from Gerri et al., 2018
kca3Tg; ubs4Tg + MO1-jam2a			Fig. S7 from Kobayashi et al., 2014
kca3Tg; umk4Tg		Fig. 6 from Taylor et al., 2015	Fig. 6 from Taylor et al., 2015
kca3Tg; vu12Tg			Fig. 5 from Shin et al., 2007
kca3Tg; vu22Tg			Fig. 7 from Ozbudak et al., 2008
kca3Tg; y273Tg		Fig. 5 from Swift et al., 2014	Fig. 5 from Swift et al., 2014
mib1^ta52b/ta52b; kca3Tg; kca4Tg	mib1
sox7^{hu5626/hu5626}; hu10049Tg; kca3Tg	sox7
supt16h^sd45/sd45; kca3Tg; mu101Tg	supt16h		Fig. 2 from Espanola et al., 2020

Sequence Information

Other Construct Pages

None

Citations