FIGURE

Figure 8

ID

ZDB-FIG-230502-9

Publication

Kondo et al., 2023 - The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney

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Figure 8

Forced activation of Notch recovers sinusoids in the jam1a^sd43 kidney

(A) Relative expression of Notch target genes, her2, her6, hey1, and hey2, in kdrl:Celurean⁺ Ac-LDL^high sinusoidal endothelial cells from wild-type, jam1a^sd43, or jam1a^sd43 expressing NICD. u.d., undetected. Error bars, s.d. (n = 3 for each).

(B–E) Dorsal view (B and C) and transverse section (D and E) of a jam1a^sd43 kidney expressing or not NICD under control of fli1:Gal4 induction. Blue areas outlined by the white dotted line in D and E denote the sinusoidal area. AF denotes auto-fluorescence of renal tubules.

(F) Percentages of sinusoidal area in the jam1a^sd43 kidney expressing or not NICD under control of fli1:Gal4 induction. Error bars, s.d. (n = 4 for each). ∗∗p < 0.01; ∗∗∗p < 0.001. Bars, 200 μm (B and C); 40 μm (D and E).

Expression Data

Genes:	Cerulean her2 her6 hey1 hey2
Fish:	sd24Tg f11r.1^sd43/sd43; sd24Tg f11r.1^sd43/sd43; sd24Tg; ubs4Tg f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg
Anatomical Term:	kidney sinusoidal blood vessel endothelium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f11r.1^sd43/sd43; sd24Tg f11r.1^sd43/sd43; sd24Tg; ubs4Tg f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg
Observed In:	kidney kidney sinusoidal blood vessel endothelium sinusoidal blood vessel endothelium
Stage:	Adult

Phenotype Detail

Acknowledgments

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