PUBLICATION
MAZe: a tool for mosaic analysis of gene function in zebrafish
- Authors
- Collins, R.T., Linker, C., and Lewis, J.
- ID
- ZDB-PUB-100211-23
- Date
- 2010
- Source
- Nature Methods 7(3): 219-223 (Journal)
- Registered Authors
- Lewis, Julian
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Cell Fusion
- HSP70 Heat-Shock Proteins/genetics
- Integrases/physiology
- Molecular Sequence Data
- Mosaicism*
- Myoblasts/metabolism
- Organ Specificity
- Promoter Regions, Genetic
- Recombination, Genetic
- Transgenes*
- Zebrafish/genetics*
- PubMed
- 20139970 Full text @ Nat. Methods
Citation
Collins, R.T., Linker, C., and Lewis, J. (2010) MAZe: a tool for mosaic analysis of gene function in zebrafish. Nature Methods. 7(3):219-223.
Abstract
To trace cell lineages in a developing vertebrate and to observe, in vivo, how behaviors of individual cells are affected by the genes they express, we created a zebrafish line containing a transgene called mosaic analysis in zebrafish (MAZe), built around a self-excising hsp70:Cre cassette. Heat shock triggers Cre recombinase-mediated recombination in a random subset of cells, bringing the transcriptional activator Gal4:VP16 under control of the EF1alpha promoter. Gal4-VP16 then activates expression of a fluorescent protein from an upstream activating sequence (UAS) promoter. Marked clones of cells expressing any desired gene product can be generated by crossing MAZe fish with other lines containing UAS-driven transgenes. The number of clones induced, and their time of origin, could be varied by adjusting heat-shock timing and duration. As an alternative to heat shock, we introduced Cre under a tissue-specific promoter in MAZe fish to generate clones in a designated tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping