FIGURE

Fig. 2

ID

ZDB-FIG-150505-4

Publication

Lin et al., 2015 - Angiopoietin-like proteins stimulate HSPC development through interaction with Notch receptor signaling

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Fig. 2

Angptls genetically interact with notch.

(A) Notch eGFP Tg reporter embryos injected with angptls-MO (2 ng) had decreased notch activity in ISV (black arrowheads) and DA (red arrowhead) at 28hpf. (B) Overexpression of constitutively active notch in angptls-MO injected (2 ng) Tg(hsp70:Gal4;UAS:NICD) can restore cmyb-positive HSPCs (arrows) at 36hpf after heatshock. (C) Mib had no cmyb-positive HSPCs (arrows) at 36hpf compared to WT or het siblings. Overexpression of zangptl2 in mib by crossing Tg(hsp70:zangptl2) into mib can restore this defect upon heatshock. Scale bars: 50 µm.

Expression Data

Genes:	EGFP myb
Fish:	WT mib1^ta52b/ta52b um14Tg cz3328Tg kca3Tg; kca4Tg WT + MO1-angptl1a + MO2-angptl2b um14Tg + MO1-angptl1a + MO2-angptl2b mib1^ta52b/ta52b; cz3328Tg kca3Tg; kca4Tg + MO1-angptl1a + MO2-angptl2b
Condition:	heat shock
Knockdown Reagents:	MO1-angptl1a MO2-angptl2b
Anatomical Terms:	dorsal aorta intersegmental vessel ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^ta52b/ta52b cz3328Tg kca3Tg; kca4Tg WT + MO1-angptl1a + MO2-angptl2b um14Tg + MO1-angptl1a + MO2-angptl2b mib1^ta52b/ta52b; cz3328Tg kca3Tg; kca4Tg + MO1-angptl1a + MO2-angptl2b
Condition:	heat shock
Knockdown Reagents:	MO1-angptl1a MO2-angptl2b
Observed In:	dorsal aorta intersegmental vessel ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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