FIGURE

Fig. 2

ID

ZDB-FIG-091215-65

Publication

Lee et al., 2009 - Notch Signaling Functions as a Cell-Fate Switch between the Endothelial and Hematopoietic Lineages

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Fig. 2

Notch Signaling Positively Regulates the Number of Hematopoietic Cells during Zebrafish Development

(A) Whole-mount RNA in situ hybridization showing the expression of gata1 in 18 hpf DMSO-treated or DAPT-treated embryos. Two additional markers, krox20 and otx1, which label the hindbrain and neuroectoderm, respectively, were used as positive controls. Notice the reduced expression of gata1 in DAPT-treated embryos (black arrow).

(B) Transverse sections of 18 hpf DMSO- or DAPT-treated embryos visualized for TOPRO (white), gata1:GFP (green), and phalloidin (red).

(C) Quantification of the hematopoietic nuclei per focal plane in DMSO-treated (n = 27) or DAPT-treated (n = 19) embryos.

(D) Transverse sections of 18 hpf phenotypic wild-type siblings and Tg(hsp70l:Gal4)^kca4;Tg(UAS:myc-NICD)^kca3 embryos visualized for TOPRO (white), gata1:GFP (green), and c-Myc as a surrogate measure for NICD expression (red).

(E) Quantification of the hematopoietic nuclei per focal plane in phenotypic wild-type siblings (n = 28) and NICD-overexpressing embryos (n = 23). DAPT-treated embryos contained 4.89 (s = 2.15) erythrocyte nuclei, in comparison to 8.07 (s = 2.38) nuclei in DMSO-treated embryos (ANOVA, p < 10^-4). Conversely, embryos overexpressing the NICD contained 9.89 (s = 2.53) erythrocyte nuclei, in comparison to 6.72 (s = 1.43) nuclei per section in phenotypic wild-type siblings (ANOVA, p < 10^-6), indicating that Notch signaling positively modulates the number of hematopoietic cells.

Expression Data

Genes:	EGFP GFP
Fish:	la781Tg kca3Tg; kca4Tg; la781Tg
Conditions:	chemical treatment: DAPT heat shock
Anatomical Term:	nucleate erythrocyte
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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