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Jag2b activates Notch signaling in somites. (A-F) Time course of Tp1:GFP (a Notch reporter) expression in the somites of wild-type embryos. Dorsal views of the embryo are shown. Yellow arrowheads indicate Tp1:GFP expression in the somites. (G) Expression of jag2b and Tp1:GFP in the somites at 13 hpf. Black and yellow arrowheads indicate jag2b- and Tp1:GFP-expressing cells, respectively. A high-magnification view of the boxed region is shown on the right. (H) Expression of Tp1:GFP in the somites of wild-type and jag2bsgRNA embryos. Yellow arrowheads indicate Tp1:GFP-expressing somites. (I) Expression of runx1 in the DA of wild-type and jag2bsgRNA embryos expressing (+) or not expressing (−) NICD under the control of phldb1:Gal4 induction. Black arrowheads indicate runx1 expression in the DA. Graph shows the distribution of embryos exhibiting ‘high’, ‘middle’ or ‘low’ runx1 expression phenotypes. Scale bars: 100 μm (A-F,I); 10 μm (G); 50 μm (H).
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