Figure 7
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Figure 7
Mafba deficiency causes G0/G1 cell cycle arrest and activates the p53 pathway. (A) The upregulation of p53 pathway genes in mafba mutants at 4 dpf was detected by qRT-PCR. (B) The cell cycle phase distribution was performed by flow cytometry at 4 dpf. The proportion of sibling and mafba−/− embryos in the G0/G1, S, and G2/M phase cell numbers. The β-actin served as endogenous control. (C) Quantitative RT-PCR analysis of the primarily relevant cell cycle factors in sibling and mafba−/− mutant embryos at 4 dpf. (D) The key cell cycle factors controlling G1/S phase transition were analyzed via RT-qPCR at 4 dpf. (E) Western blot analysis of CDK2, CDK6, Cyclin D1, Cyclin E1, and Pcna at 4 dpf. GAPDH was used to normalize the protein. Data are mean ± SD; ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the sibling control (by ANOVA followed by Dunnett’s multiple comparison).