Figure 8—figure supplement 1.

Elevation of pERK levels in phorbol 12-myristate 13-acetate (PMA)-treated and <italic toggle='yes'>hai1a</italic> mutant epidermis.

(A–J) Lateral projected confocal images of ventral trunks (A–C), tails (D–F), and whole embryos (G–L) immunostained for TP63 (magenta) and pERK (green) at 24hpf (A–D, K, L), 48hpf (E, F), 16hpf (G, H), and 20hpf (I, J). Both hai1afr26 (C, D, F, H, J, L) and 125 ng/ml PMA-treated (B) embryos show increased epidermal pERK levels compared to untreated WT (A, E, G, I, K). Elevation of epidermal pERK is seen in hai1afr26 mutants and PMA treated embryos as well as in nascent aggregates (arrowheads, H, J). (M, N) Lateral projected confocal images of the trunks of WT (M) and hai1afr26 (N) embryos immunostained with total ERK (tERK; green) and TP63 (magenta). There was no change in tERK in hai1afr26 (N) compared to WT (M). Scale bars: (C, F, N) = 50 µm; (D) = 20 µm; (H, J, K, L) = 100 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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