Figure 7
- ID
- ZDB-FIG-210725-91
- Publication
- Ma et al., 2021 - Matriptase activation of Gq drives epithelial disruption and inflammation via RSK and DUOX
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(A, B) Confocal images of the ventral fin of 22hpf sibling (A) or hai1ahi2217 (B) embryos injected with RNA encoding PKCδ-GFP. Mostly cytoplasmic distribution in sibling was relocated to cell and nuclear membranes in hai1a mutants. (C, D) Lateral brightfield images of 48hpf hai1ahi2217 larvae treated with 0.5% DMSO (C) or 85 µM GFX109203 (D). Epidermal aggregates and fin deterioration are rescued by the PKC inhibitor (arrowheads). (E–H) DIC (E, G) and projected confocal images (E′, G′, F, H) of hai1ahi2217; Tg(mpx:eGFP)i114 trunk at 24hpf (E–E′, G–G′) and tail at 48hpf (F, H), either treated with 0.5% DMSO (E–F) or 85 µM GFX109203 (G–H). Embryos are immunostained for TP63 (magenta) and eGFP (green), highlighting rescue of epidermal phenotype and partial rescue of neutrophils by GFX109203. (I) Counts of eGFP-positive neutrophils in the fins at 48hpf of Tg(mpx:eGFP)i114, or hai1ahi2217; Tg(mpx:eGFP)i114 treated with 0.5% DMSO or 85 µM GFX109203. n = 8; ANOVA, Dunn’s multiple comparisons; **p<0.01. Scale bars: (A) = 10 µm; (D) = 200 µm; (H) = 100 µm. |