ZFIN Image: Ma et al., 2021, Figure 8

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Figure Caption

Figure 8—figure supplement 1. Elevation of pERK levels in phorbol 12-myristate 13-acetate (PMA)-treated and <italic toggle='yes'>hai1a</italic> mutant epidermis.

(A–J) Lateral projected confocal images of ventral trunks (A–C), tails (D–F), and whole embryos (G–L) immunostained for TP63 (magenta) and pERK (green) at 24hpf (A–D, K, L), 48hpf (E, F), 16hpf (G, H), and 20hpf (I, J). Both hai1a^fr26 (C, D, F, H, J, L) and 125 ng/ml PMA-treated (B) embryos show increased epidermal pERK levels compared to untreated WT (A, E, G, I, K). Elevation of epidermal pERK is seen in hai1a^fr26 mutants and PMA treated embryos as well as in nascent aggregates (arrowheads, H, J). (M, N) Lateral projected confocal images of the trunks of WT (M) and hai1a^fr26 (N) embryos immunostained with total ERK (tERK; green) and TP63 (magenta). There was no change in tERK in hai1a^fr26 (N) compared to WT (M). Scale bars: (C, F, N) = 50 µm; (D) = 20 µm; (H, J, K, L) = 100 µm.

Acknowledgments

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