FIGURE

Fig. S9

ID

ZDB-FIG-110322-67

Publication

van der Velden et al., 2011 - The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish

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Fig. S9

The lkb1-mutant larvae are not hypoxic. Whole-mount in situ hybridization for expression of the hypoxia-inducible factor 1 subunit alpha (HIF1α) target genes ldha and ndufa4 in WT (A and D), lkb1-mutant (B and E) and von Hippel–Lindau (vhl)-mutant (C and F) embryos. Lateral views, anterior to the left. (A) WT embryo at 7 dpf stained for ldha1; expression is detected in the brain. The same pattern is observed in lkb1 embryos at 7 dpf (B), whereas vhl-mutant embryos that exhibit constitutive activation of the hypoxia program show up-regulation of ldha1 expression throughout the embryo (C). (D) WT embryo at 7 dpf showing expression of ndufa4 in the brain. The same pattern is observed in lkb1-mutant embryos at 7 dpf, whereas vhl-mutant embryos show widespread up-regulation of ndufa4 expression.

Expression Data

Genes:	ldha ndufa4b
Fish:	WT stk11^{hu1960/hu1960} vhl^{hu2117/hu2117}
Anatomical Terms:	brain whole organism
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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