ZFIN Image: van der Velden et al., 2011, Fig. S9

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Figure Caption

Fig. S9

The lkb1-mutant larvae are not hypoxic. Whole-mount in situ hybridization for expression of the hypoxia-inducible factor 1 subunit alpha (HIF1α) target genes ldha and ndufa4 in WT (A and D), lkb1-mutant (B and E) and von Hippel–Lindau (vhl)-mutant (C and F) embryos. Lateral views, anterior to the left. (A) WT embryo at 7 dpf stained for ldha1; expression is detected in the brain. The same pattern is observed in lkb1 embryos at 7 dpf (B), whereas vhl-mutant embryos that exhibit constitutive activation of the hypoxia program show up-regulation of ldha1 expression throughout the embryo (C). (D) WT embryo at 7 dpf showing expression of ndufa4 in the brain. The same pattern is observed in lkb1-mutant embryos at 7 dpf, whereas vhl-mutant embryos show widespread up-regulation of ndufa4 expression.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA