FIGURE

Fig. S5

ID

ZDB-FIG-110322-63

Publication

van der Velden et al., 2011 - The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish

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Fig. S5

Zebrafish TOR (zTOR) signaling is not grossly deregulated in lkb1-mutant intestine. Transverse sections of WT and lkb1-mutant intestines at indicated days of development were stained with an antibody against phospho-S6 ribosomal protein (RS6, Ser240/244) (A–E) and phospho-4EBP1 (Thr-37/46) (F–J). Strong phospho-RS6 staining is detected in WT and lkb1-mutant intestines at 5 dpf (A and B). Food-deprived WT larvae show moderate staining at 7 and 11 dpf (C and E). Staining in lkb1 mutants (B and D) is not overtly different from staining in age-matched WT larvae (A and C). Moderate phospho-4EBP1 stating is detected in WT and lkb1-mutant intestines at 5 dpf. (F and G). Food-deprived WT larvae show decreased staining at 7 and 11 dpf (H and J). Staining in lkb1 mutants is similar to that in age-matched WT larvae (G and I). Red arrows indicate expression in the pancreas.

Expression Data

Antibodies:	Ab1-eif4ebp1 Ab1-rps6
Fish:	WT stk11^{hu1960/hu1960}
Condition:	fasting
Anatomical Term:	intestine
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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