FIGURE

Fig. 4

ID

ZDB-FIG-110322-57

Publication

van der Velden et al., 2011 - The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish

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Fig. 4

The zebrafish TOR (zTOR) pathway activity in lkb1 mutants resembles that in starved WT larvae. (A) Western blot analysis with antibodies against phospho-AMPK and phospho-ACC of total protein lysates from embryos at the indicated days and genotypes. AMPK and ACC are not phosphorylated in WT or lkb1-mutant larvae at 5 or 7 dpf. Prolonged fasting induces phosphorylation of AMPK and ACC in 11-dpf WT larvae. Elevated expression of AMPK in 7-dpf lkb1-mutant larvae is detected. (B) Western blot analysis using anti–phospho-p70S6K, anti-p70S6K, anti–phospho-4EBP1, anti-4EBP1, anti–phospho-RS6, and anti-RS6 antibodies of total protein lysates from embryos at the indicated days and genotypes. (C) Sections of livers obtained from WT and lkb1-mutant embryos at the indicated days stained with anti–phospho-RS6 and anti–phospho-4EBP1.

Expression Data

Antibodies:	Ab1-acac Ab1-eif4ebp1 Ab1-prkaa Ab1-rps6 Ab2-acac Ab2-eif4ebp1 Ab2-prkaa Ab3-rps6 Ab8-rps6k Ab9-rps6k
Fish:	WT stk11^{hu1960/hu1960}
Condition:	fasting
Anatomical Terms:	liver whole organism
Stage Range:	Long-pec to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stk11^{hu1960/hu1960} stk11^{hu1968/hu1968}
Observed In:	TOR signaling
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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