Gene Trap Construct

Gt(GAL4FF)

ID

ZDB-GTCONSTRCT-080520-2

Name

Gt(GAL4FF)

Previous Names

Gt(Gal4FF)
T2KSAGFF (1)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

GAL4FF (1)

Contains Sequences

T2K (1)

Note

Gene trap construct with DNA-binding domain from Gal4 and two transcription activating modules from VP16 fused to medaka Tol2 transposable element. The construct also feature a splice acceptor from the rabbit beta-globin gene.

Plasmid Map

Genomic Features

That Utilize Gt(GAL4FF) Construct

Genomic Feature	Affected Genomic Regions
mpn302Gt
mpn309Gt
mpn317Gt
mpn318Gt
mpn323Gt
mpn326Gt
mpn354Gt
nk128aGt
nk158aGt
nk168aGt
nk193aGt
nk206aGt
nk226FGt
nk233AGt
nk234AGt
nk31bGt
nk36bGt
nk73aGt
nkgSAGFF138AGt
nkgSAGFFf279AGt
nksagff120ATg
nkSAGFF155AGt
nksagff179aGt
nksagff187aGt
nksagff202aTg	erbb2
nksagff212cGt
nksagff214aGt
nksagff228ATg
nksagff228cGt
nksagff231ATg
nksagff234aGt
nksagff234DTg
nksagff27aGt
nksagff27cGt	map3k22
nksagff70ATg
nksagff81BGt
nksagff91bGt
rw0319Gt

Transgenics

That Utilize Gt(GAL4FF) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
c264Tg; nksagff214aGt (EKW)
c264Tg; nksagff214aGt; pd1151Tg (EKW)		Fig. 3 from Garcia et al., 2017	2 figures from Garcia et al., 2017
cavin1b^{bns110/bns110}; c264Tg; nksagff214aGt (EKW)	cavin1b
cavin1b^{bns110/bns110}; c264Tg; nksagff214aGt; pd1151Tg (EKW)	cavin1b	Fig. 2 from Garcia et al., 2017	Fig. 2 from Garcia et al., 2017
cfdp1^nub36/nub36; nk73aGt; nkUAS:Gtuba2Tg	cfdp1	Fig. 9 from Itoh et al., 2021	Fig. 9 from Itoh et al., 2021
dstyk^s739/s739; nksagff214aGt; nkuasgfp1aTg	dstyk	Fig. 8 from Bagwell et al., 2020	Fig. 8 from Bagwell et al., 2020
dstyk^s739/s739; nksagff214aGt; nkuasgfp1aTg; pd46Tg	dstyk	Fig. 7 from Bagwell et al., 2020	Fig. 7 from Bagwell et al., 2020
enpp1^{hu4581/hu4581}; hu8811Tg; nksagff214aGt	enpp1	Fig. 5 from Apschner et al., 2014
erbb2^{nksagff202aTg/+}; bc3Tg	erbb2		Fig. 6 from Xiao et al., 2015
erbb2^{nksagff202aTg/+}; bc3Tg; nkuasgfp1aTg	erbb2		Fig. 2 from Xiao et al., 2015
erbb2^{nksagff202aTg/nksagff202aTg}; bc3Tg	erbb2	Fig. 6 from Xiao et al., 2015	Fig. 6 from Xiao et al., 2015
erbb2^{nksagff202aTg/nksagff202aTg}; bc3Tg; nkuasgfp1aTg	erbb2	Fig. 2 from Xiao et al., 2015	Fig. 2 from Xiao et al., 2015
erbb2^{nksagff202aTg}; bc3Tg; hzm11Tg	erbb2		2 figures from Xiao et al., 2015
erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg	erbb2	2 figures from Tian et al., 2020	5 figures from 2 publications
erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg; s356tTg	erbb2	Fig. 7 from Xiao et al., 2015	Fig. 7 from Xiao et al., 2015
erbb2^{nksagff202aTg}; nkuasgfp1aTg	erbb2		Fig. 1 from Xiao et al., 2015
erbb2^{nksagff202aTg}; sarm1^hzm13/hzm13; bc3Tg; nkuasgfp1aTg	erbb2 sarm1	3 figures from Tian et al., 2020	Fig. 5 from Tian et al., 2020
erbb2^st61/st61; erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg	erbb2	Fig. 6 from Tian et al., 2020
erbb2^st61/st61; erbb2^{nksagff202aTg}; sarm1^hzm13/hzm13; bc3Tg; nkuasgfp1aTg	erbb2 sarm1	Fig. 6 from Tian et al., 2020
etsrp^{uq13ks/uq13ks}; map3k22^{nksagff27cGt/nksagff27cGt}	etsrp map3k22		2 figures from Capon et al., 2022
etsrp^{uq13ks/uq13ks}; map3k22^{nksagff27cGt/nksagff27cGt}; fr13Tg/fr13Tg	etsrp map3k22		Fig. 7 from Capon et al., 2022
etsrp^{uq13ks/uq13ks}; map3k22^{nksagff27cGt/nksagff27cGt}; fr14Tg/fr14Tg	etsrp map3k22
hu7478Tg; nksagff214aGt; nkuasgfp1aTg			Fig. 3 from LLeras Forero et al., 2018
kca33Tg; nksagff214aGt			Fig. 1 from Ellis et al., 2013
ko112Tg; nk73aGt			Fig. 4 from Iida et al., 2018
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg	map3k22		3 figures from Bussmann et al., 2010
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl		Fig. 4 from Bussmann et al., 2010	Fig. 4 from Bussmann et al., 2010
map3k22^{nksagff27cGt/+}; nkuasgfp1aTg	map3k22		Fig. 1 from Bussmann et al., 2010
map3k22^{nksagff27cGt/nksagff27cGt}	map3k22	Fig. 2 from Capon et al., 2022	3 figures from Capon et al., 2022
map3k22^{nksagff27cGt/nksagff27cGt}; fr13Tg/fr13Tg	map3k22		Fig. 7 from Capon et al., 2022
map3k22^{nksagff27cGt/nksagff27cGt}; fr14Tg/fr14Tg	map3k22		Fig. 7 from Capon et al., 2022
map3k22^{nksagff27cGt/nksagff27cGt}; s916Tg/s916Tg	map3k22		Fig. 1 from Capon et al., 2022
map3k22^nksagff27cGt; ncv1Tg; nkuasgfp1aTg + MO1-svep1		Fig. S5 from Morooka et al., 2017
map3k22^nksagff27cGt; nkuasgfp1aTg	map3k22	Fig. 1 from Ernens et al., 2017	2 figures from Ernens et al., 2017
mde10Tg; nk73aGt		2 figures from Ohki et al., 2017
mde11Tg; nk73aGt		Fig. 3 from Ohki et al., 2017
mde11Tg; nk73aGt; s896Tg		Fig. S5 from Ohki et al., 2017
mde12Tg; nk73aGt		Fig. 3 from Ohki et al., 2017
mde8Tg; nk73aGt		2 figures from Ohki et al., 2017
mde9Tg; nk73aGt		2 figures from Ohki et al., 2017
mw33Tg; nksagff214aGt		3 figures from Ellis et al., 2013	Fig. 3 from Ellis et al., 2013
nk128aGt; nkuasgfp1aTg			Fig. S6 from Asakawa et al., 2008
nk128aGt; nkuastetxlccfp6aTg		text only from Asakawa et al., 2008
nk158aGt; nkuasgfp1aTg			Fig. S6 from Asakawa et al., 2008
nk158aGt; nkuasrfp1aTg
nk158aGt; nkuastetxlccfp6aTg		text only from Asakawa et al., 2008
nk168aGt; nkuasgfp1aTg			2 figures from Imai et al., 2010
nk168aGt; nkuasgfp1aTg + MO1-fzr1a		Fig. 8 from Imai et al., 2010	Fig. 8 from Imai et al., 2010
nk168aGt; nkuasgfp1aTg + MO1-psmd6		Fig. 8 from Imai et al., 2010	Fig. 8 from Imai et al., 2010
nk193aGt; nkuasgfp1aTg			Fig. S6 from Asakawa et al., 2008
nk193aGt; nkuastetxlccfp6aTg		text only from Asakawa et al., 2008
nk206aGt; nkuasgfp1aTg			Fig. S6 from Asakawa et al., 2008
nk206aGt; nkuastetxlccfp6aTg		text only from Asakawa et al., 2008
nk31bGt/+; nkuasgfp1aTg			Fig. 4 from Kishimoto et al., 2013
nk31bGt/nk31bGt; nkuasgfp1aTg		Fig. 4 from Kishimoto et al., 2013	Fig. 4 from Kishimoto et al., 2013
nk31bGt; nkuasgfp1aTg			5 figures from 2 publications
nk31bGt; nkuasgfp1aTg; ups1Tg			Fig. 5 from Kishimoto et al., 2013
nk31bGt; nkuastetxlc13aTg
nk31bGt; nkuastetxlccfp6aTg		Fig. 3 from Asakawa et al., 2008	Fig. 4 from Asakawa et al., 2008
nk36bGt
nk36bGt; nkuasgfp1aTg			2 figures from Asakawa et al., 2008
nk36bGt; nkuastetxlc13aTg
nk36bGt; nkuastetxlccfp6aTg		Fig. 3 from Asakawa et al., 2008	Fig. 4 from Asakawa et al., 2008
nk73aGt (EKW)			Fig. S2 from Trivellin et al., 2020
nk73aGt; nkGCaMPHS4aTg
nk73aGt; nkUAS:Gtuba2Tg			Fig. 9 from Itoh et al., 2021
nk73aGt; nksaigff213aGt/+; nkuasmrfp1cry2oligTg
nk73aGt; nkuasgfp1aTg			Fig. S6 from Asakawa et al., 2008
nk73aGt; nkuasmrfp1cry2oligTg			Fig. 2 from Asakawa et al., 2020
nk73aGt; nkuasoptdp43zTg			Fig. 2 from Asakawa et al., 2020
nk73aGt; nkuastetxlccfp6aTg		text only from Asakawa et al., 2008
nk73aGt; s2504Tg		Fig. 2 from Dong et al., 2013	Fig. 2 from Dong et al., 2013
nk73aGt; y672Tg (EKW)			Fig. S2 from Trivellin et al., 2020
nk73aGt; zf424Tg		Fig. 2 from Wada et al., 2013
nk73aGt; zf424Tg + MO4-kremen1		Fig. 2 from Wada et al., 2013
nk73aGt; zf425Tg		Fig. 2 from Wada et al., 2013
nk73aGt; zf507Tg			Fig. 4 from Sato et al., 2013
nkSAGFF155AGt		Fig. 3 from Wai et al., 2015	Fig. 3 from Wai et al., 2015
nkgSAGFF138AGt; zf2166Tg			Fig. 1 from Enya et al., 2018
nksagff179aGt; nkuasgfp1aTg/+			2 figures from Koide et al., 2009
nksagff187aGt; nkuasgfp1aTg
nksagff214aGt
nksagff214aGt; nkuasgfp1aTg			Fig. 8 from Bagwell et al., 2020
nksagff214aGt; nkuasgfp1aTg; pd46Tg			2 figures from Bagwell et al., 2020
nksagff214aGt; nkuasrfp1aTg/+
nksagff228cGt; nkuasgfp1aTg/+			Fig. 1 from Koide et al., 2009
nksagff234aGt; nkuasgfp1aTg			Fig. S2 from Heanue et al., 2016
nksagff27aGt; nkuasgfp1aTg/+			2 figures from Koide et al., 2009
nksagff27aGt; nkuastetxlccfp6aTg		Fig. 1 from Koide et al., 2009
nksagff91bGt; nkuasgfp1aTg/+			2 figures from Koide et al., 2009
npas4l^{uq14ks/uq14ks}; map3k22^{nksagff27cGt/nksagff27cGt}	map3k22 npas4l		Fig. 4 from Capon et al., 2022
prox3^sa35/sa35; map3k22^nksagff27cGt; nkuasgfp1aTg	map3k22 prox3
psmd6^rw619/rw619; nk168aGt; nkuasgfp1aTg	psmd6	Fig. 3 from Imai et al., 2010	Fig. 3 from Imai et al., 2010
ret^hu2846/+; nksagff234aGt; nkuasgfp1aTg	ret	Fig. 2 from Heanue et al., 2016	Fig. S2 from Heanue et al., 2016
rw0319Gt
vangl2^tc240/tc240; nksagff187aGt; nkuasgfp1aTg; rw0Tg	vangl2	Fig. 3 from Sittaramane et al., 2013	Fig. 3 from Sittaramane et al., 2013
vangl2^tc240/tc240; nksagff187aGt; nkuasgfp1aTg; rw0Tg; zou001Tg	vangl2	Fig. 3 from Sittaramane et al., 2013	Fig. 3 from Sittaramane et al., 2013

Sequence Information

Other Construct Pages

None

Citations