FIGURE

Fig. S2

ID

ZDB-FIG-170222-37

Publication

Heanue et al., 2016 - A Novel Zebrafish ret Heterozygous Model of Hirschsprung Disease Identifies a Functional Role for mapk10 as a Modifier of Enteric Nervous System Phenotype Severity

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Fig. S2

Novel transgenic tools allow labeling of ENS progenitors and ENS neurons.

SAGFF234A is a transgenic line with the Gal4 expressing gene trap construct integrated within the chrm2a gene on the chromosome 4 encoding the cholinergic receptor, muscarinic 2a. When crossed to transgenic fish carrying the GFP reporter gene downstream of the Gal4 recognition sequence (UAS:GFP) generates transgenic embryos expressing GFP within the ENS at 4dpf (A, B), in both ENS progenitors at 40hpf (C, arrows) and in virtually all HuC/D⁺ ENS neurons at 4dpf (D). (E) At 7dpf GFP can be detected in cellular processes of HuC/D⁺ ENS neurons, distal intestine shown (cellular processes of ENS neurons indicated with arrows). The high-resolution image of the full gut length (in D) was constructed by tiling two overlapping micrographs.

Expression Data

Gene:	EGFP
Fish:	nksagff234aGt; nkuasgfp1aTg ret^hu2846/+; nksagff234aGt; nkuasgfp1aTg
Anatomical Terms:	enteric nervous system enteric neuron enteric neuron cell projection presumptive enteric nervous system
Stage Range:	Prim-25 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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