FIGURE

Fig. 5

ID

ZDB-FIG-200207-5

Publication

Tian et al., 2020 - Systemic loss of Sarm1 protects Schwann cells from chemotoxicity by delaying axon degeneration

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Fig. 5

Schwann cells develop normally in Sarm1-deficient zebrafish.

a Confocal images of a double-transgenic 5dpf larva showing Schwann cells marked by expression of GFP (green) under the control of the Tg[gSAGFF202A] Gal4 driver, and lateralis afferent neurons marked by expression of mCherry under the control of the SILL enhancer (red). Wild type (top), Sarm1 mutants (bottom). Scale bar 20 μm. b Images show the indicated time points after axon transection (hours post-injury = hpi) from a videomicroscopic recording of Schwann cells (green) and their interaction with axons (red) in wild type and Sarm1−/−. White arrowheads indicate Schwann cells engulfing axonal debris in the wild type. A white arrow indicates degradation-resistant axon segment in Sarm1−/−. Please note that the proximal axon stump in Sarm1−/− is not visible in these images because it is outside the focal plane.

Expression Data

Genes:	EGFP mCherry
Fish:	erbb2^{nksagff202aTg}; bc3Tg; nkuasgfp1aTg erbb2^{nksagff202aTg}; sarm1^hzm13/hzm13; bc3Tg; nkuasgfp1aTg
Condition:	transection: axon: posterior lateral line nerve
Anatomical Terms:	lateral line nerve axon myelinating Schwann cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	erbb2^{nksagff202aTg}; sarm1^hzm13/hzm13; bc3Tg; nkuasgfp1aTg
Observed In:	Schwann cell development
Stage:	Day 5

Phenotype Detail

Acknowledgments

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