FIGURE

Fig. 2

ID

ZDB-FIG-131105-2

Publication

Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Wnt Signaling Positively Regulates Neuromast Size, Whereas Dkk2/Krm Signaling Negatively Regulates It

(A) Neuromast O2 reaches its final size at 80 hpf in wild-type embryos. By contrast, O2 reaches its final size before 48 hpf, and the number of hair cells does not change over time in dkk2-overexpressing embryos.

(B) Manipulations of Wnt signaling affect hair cell numbers. Mean ± SEM is indicated. p < 0.001 (t test).

(C–E) Knockdown of dkk2 (D) or krm (E) gene function increases neuromast size and hair cell number, as revealed by DiAsp labeling.

(F and G) Wnt inhibition by overexpression of dkk2 reduces neuromast size, number of hair cells (F), and number of dividing cells (G). Scale bar represents 20 μm.

(H) Pharmacological blockade of cell division phenocopies inhibition of Wnt signaling.

See also Figures S1–S3.

Expression Data

Genes:	EGFP RFP
Fish:	zf106Tg zf106Tg + MO2-dkk2 zf106Tg + MO4-kremen1 zf422Tg; zf424Tg
Knockdown Reagents:	MO2-dkk2 MO4-kremen1
Anatomical Term:	neuromast
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dkk1a WT + MO2-dkk2 WT + MO3-lef1 WT + MO4-kremen1 WT + MO5-dkk1b zf106Tg + MO2-dkk2 zf106Tg + MO4-kremen1 nk73aGt; zf424Tg nk73aGt; zf425Tg zf422Tg; zf423Tg zf422Tg; zf424Tg nk73aGt; zf424Tg + MO4-kremen1
Knockdown Reagents:	MO1-dkk1a MO2-dkk2 MO3-lef1 MO4-kremen1 MO5-dkk1b
Observed In:	neuromast neuromast mitotic cell cycle neuromast hair cell
Stage Range:	Prim-25 to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.