FIGURE

Figure 3

ID

ZDB-FIG-210519-76

Publication

Knickmeyer et al., 2021 - BMP Signaling Interferes with Optic Chiasm Formation and Retinal Ganglion Cell Pathfinding in Zebrafish

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Figure 3

Transcriptomic analysis of forebrain tissue after bmp4 overexpression reveals differential regulation of several axon guidance factors. (A) Scheme of sample collection for transcriptome analysis. (B,C) Expression changes of selected genes related to (B) optic cup patterning and BMP signaling, and (C) axon guidance, in tg(hsp70l:bmp4) embryos induced at 15 hpf, normalized to controls, as determined by microarray at 21 hpf. Expression level change displayed as log2(Fold change). * adj. p < 0.05, ** adj. p < 0.01, *** adj. p < 0.001. (D–O) In situ hybridization of genes related to axon guidance at 21 hpf in tg(hsp70l:bmp4) embryos and controls induced at 15 hpf. (D,E) sema3d. (F,G) sema3ab. (H,I) lrig1. (J,K) ephb2b. (L–O) rgmd. (D–G,J,K,N,O) Sagittal view, nasal to the left; (H,I,L,M) transverse view. All images are maximum intensity projections; counterstaining with DAPI, scale bar 50 µm.

Expression Data

Genes:	bambia bambib bmp7a bmp7b bmpr1aa bmpr1ab bmpr1ba bmpr1bb efna1b efnb3b epha3 ephb2b fsta lrig1 nog1 nog2 plxnb1a rgma rgmd sema3ab sema3d slit3 tbx3a vax1 vax2
Fish:	WT af1Tg
Condition:	heat shock
Anatomical Terms:	diencephalon axis forebrain immature eye optic cup dorsal region optic stalk
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	af1Tg
Condition:	heat shock
Observed In:	forebrain immature eye optic cup dorsal region
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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