FIGURE

Figure 5

ID

ZDB-FIG-200725-24

Publication

Toms et al., 2020 - Clinical and preclinical therapeutic outcome metrics for USH2A-related disease

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Figure 5

Generation of ush2a^u507zebrafish. (A) Schematic representation of zebrafish and human usherin domain architecture. The u507 mark on zebrafish usherin indicates the region targeted by CRISPR/Cas9. (B) Sanger sequencing traces showed the ush2a gene sequence change in homozygous ush2a^u507 mutant zebrafish (c.2131_2203 + 73delinsCGGCGG) at the CRISPR/Cas9 target site. (C) Anti-usherin-C (red) and anti-centrin (green) immunostaining of retinal sections from wt and ush2a^u507 larvae (5 dpf). DAPI nucleic acid stain was used as a counterstain (blue). PAM, protospacer adjacent motif. INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; dpf, days post fertilization. Scale bar = 10 μm.

Expression Data

Gene:	ush2a
Antibodies:	Ab1-cetn Ab1-ush2a
Fish:	AB ush2a^{zf3378/zf3378} (AB)
Anatomical Terms:	retina photoreceptor cell retina photoreceptor cell cilium
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ush2a^{zf3378/zf3378} (AB)
Observed In:	retina photoreceptor cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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