IMAGE

Figure 5

ID

ZDB-IMAGE-200725-24

Genes

ush2a

Antibodies

Source

Figures for Toms et al., 2020

Image

Figure Caption

Figure 5

Generation of ush2a^u507zebrafish. (A) Schematic representation of zebrafish and human usherin domain architecture. The u507 mark on zebrafish usherin indicates the region targeted by CRISPR/Cas9. (B) Sanger sequencing traces showed the ush2a gene sequence change in homozygous ush2a^u507 mutant zebrafish (c.2131_2203 + 73delinsCGGCGG) at the CRISPR/Cas9 target site. (C) Anti-usherin-C (red) and anti-centrin (green) immunostaining of retinal sections from wt and ush2a^u507 larvae (5 dpf). DAPI nucleic acid stain was used as a counterstain (blue). PAM, protospacer adjacent motif. INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; dpf, days post fertilization. Scale bar = 10 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.