Expression levels of apoptosis, fibrosis, and cell proliferation-related genes in adult wild-type zebrafish treated with Oligo-Fucoidan and radiation. (A) The expression of B-cell lymphoma 2 (bcl2)-associated agonist of cell death (bad), bcl2-associated X protein a (baxa), apoptosis-related cysteine peptidase 3a (caspase 3a), apoptosis-related cysteine peptidase 8 (caspase 8), apoptosis antigen 1 (fas), c-Jun N-terminal kinase 1 (jnk-1), mitogen-activated protein kinase 8a (p38(MAPK)) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40Gy). (B) The expression of collagen, type I, alpha 1a (col1a1), connective tissue growth factor a (ctgfa), heparanase (hpse) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40 Gy). (C) The expression of cyclin E1 (ccne1), cyclin-dependent kinase 1 (cdk1), and cyclin-dependent kinase 2 (cdk2) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40 Gy). (D) The expression of col1a1, ctgfa, and hpse in wild-type fish treated with 10 Gy irradiation (10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (OF + 10 Gy). The data are presented as dot plots with a horizontal line for the mean and are repeated in triplicate. The statistical significance was calculated using Student’s t-test (* p < 0.05, ** p < 0.01, ns: non-significant).
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