Figure 4

Representative images of hepatocyte apoptosis detected on hematoxylin and eosin (H&E) stain liver specimen from WT and transgenic fish with radiation without Oligo-Fucoidan or with Oligo-Fucoidan pretreatment. The images were taken at 400× magnification, and the scale shown is for 30 µm; the box area is enlarged to show the hepatocyte apoptosis, and the blue arrows pointed to the apoptotic hepatocytes. (A–C) The hepatocyte apoptosis in wild-type fish (WT) with 10 Gy irradiation (WT + 10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (WT + OF + 10 Gy). (D) Statistical analysis of hepatocyte apoptosis feature of WT fish. (E–G) The hepatocyte apoptosis in Tg (HBx, src, p53−) transgenic fish with diet-induced obesity (DIO) with 10 Gy irradiation (DIO + 10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (DIO + OF + 10 Gy). (H) Statistical analysis of hepatocyte apoptosis feature of Tg (HBx, src, p53−) fish. (I–K) The hepatocyte apoptosis in Tg (HBx, src) transgenic fish with diet-induced obesity (DIO) with 10 Gy irradiation (DIO + 40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (DIO + OF + 40 Gy). (L) Statistical analysis of hepatocyte apoptosis feature of Tg (HBx, src) fish. The data are presented as dot plots with a horizontal line for the mean and are repeated in triplicate. The statistical significance was calculated using Student’s t-test (* p < 0.05, ** p < 0.01).