Histopathological analysis by H&E staining of hepatocytes in adult HBx, src transgenic zebrafish treated with Oligo-Fucoidan and radiation. (A,B) Representative images for H&E stain were shown. The images were taken at 400× magnification, and the scale shown is for 30 µm. (A) Statistical analysis of H&E staining of the liver sections from HBx, src transgenic fish diet-induced obesity (DIO) with 40 Gy irradiation (DIO + 40 Gy) and oral feeding Oligo-Fucoidan (DIO + OF + 40 Gy). (B) Statistical analysis of H&E staining of the liver sections from HBx,src,p53-transgenic fish diet-induced obesity (DIO) with 10 Gy irradiation (DIO + 10 Gy) and oral feeding Oligo-Fucoidan (DIO + OF + 10 Gy). The different colors denote the different pathological features as follows: gray: normal, green: steatosis, yellow: hyperplasia, orange: dysplasia, and red: hepatocellular carcinoma (HCC). N = 4–5 for the DIO group, and N = 9–10 for the other groups. (C,D) Representative images for proliferating cell nuclear antigen (PCNA) immunohistochemistry (IHC) stain were shown. The images were taken at 400× magnification, and the scale shown is for 30 µm. (C) Statistical analysis of PCNA immunohistochemistry staining of the liver sections from HBx,src transgenic fish diet-induced obesity (DIO) with 40 Gy irradiation (DIO + 40 Gy) and oral feeding Oligo-Fucoidan (DIO + OF + 40 Gy). (D) Statistical analysis of PCNA IHC staining of the liver sections from HBx,src,p53-transgenic fish diet-induced obesity (DIO) with 10 Gy irradiation (DIO + 10 Gy) and oral feeding Oligo-Fucoidan (DIO + OF + 10 Gy). The data are presented as dot plots with a horizontal line for the mean and are repeated in triplicate. The statistical significance was calculated using Student’s t-test (**** p < 0.0001).
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