FIGURE

Fig. 1

ID

ZDB-FIG-180823-43

Publication

Ghosh et al., 2018 - Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

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Fig. 1

PG1 hox function is not required for expression of most r4 genes. Expression of r4 genes was assayed via ISH in (i) WT, (ii) hoxb1b mutant, (iii) hoxb1a mutant, (iv) hnf1ba mutant and (v) valentino mutant zebrafish. The genes assayed include a hoxb1a, b dusp2, c dusp6, d spry1, e fgf3, f fgf8, g efnb2a, h meis1a, i ccnjl, j irx7, k sall4, l greb1l, m egfl6, n hoxb2a and o engrailed1b. krox20 (red) which is expressed in r3 and r5, was used to assign the expression domains of several genes, as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging. Black arrows point to the r4 domain in 12hpf and 24hpf embryos. White brackets mark the normal and expanded r4 domains in embryos staged at 14hpf, 16hpf and 19 hpf

Expression Data

Genes:	ccnj dusp2 dusp6 efnb2a egfl6 egr2b en1b fgf3 fgf8a greb1l hoxb1a hoxb2a irx7 meis1a sall4 spry1
Fish:	WT hnf1ba^{hi2169Tg/hi2169Tg} hoxb1a^um191/um191 hoxb1b^um197/um197 mafba^b337/b337
Anatomical Terms:	presumptive rhombomere 3 presumptive rhombomere 4 rhombomere 4
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hnf1ba^{hi2169Tg/hi2169Tg} hoxb1a^um191/um191 hoxb1b^um197/um197
Observed In:	rhombomere 4 rhombomere 4 Mauthner neuron
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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