FIGURE

Fig. 3

ID

ZDB-FIG-180823-46

Publication

Ghosh et al., 2018 - Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

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Fig. 3

Expression of the r5/r6 gene-set is dependent on hnf1ba and valentino. Expression of r5/r6 candidate genes was assayed via ISH in (i) WT, (ii) hoxb1b mutant, (iii) hoxb1a mutant, (iv) hnf1ba mutant and (v) valentino mutant zebrafish lines. The genes assayed include a tox3, b sema3fb, c mpz, d gas6, e celf2, f nr2f2, g col15a1b and h gbx1. krox20 (red), which is expressed in r3 and r5, was used to position the expression domains of several genes as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging. Black brackets mark the smaller r5 domain. Red bracket in (Hiii) indicates expression of gbx1 throughout the hindbrain, as a result of reappearance of gbx1 expression in r4 of hoxb1a mutants

Expression Data

Genes:	celf2 egr2b gas6 gbx1 mpz nr2f2 sema3fb tox3
Fish:	WT hnf1ba^{hi2169Tg/hi2169Tg} hoxb1a^um191/um191 hoxb1b^um197/um197 mafba^b337/b337
Anatomical Terms:	rhombomere 1 rhombomere 2 rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 6
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hnf1ba^{hi2169Tg/hi2169Tg} hoxb1a^um191/um191 mafba^b337/b337
Observed In:	rhombomere 1 rhombomere 2 rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 6
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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