FIGURE

Fig. 4

ID

ZDB-FIG-180823-47

Publication

Ghosh et al., 2018 - Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

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Fig. 4

Expression of the r5/r6 gene-set requires FGF and RA signaling. Expression of r5/r6 genes was assayed via ISH in (i) WT embryos treated with 50uM DMSO, (ii) WT embryos treated with 50uM SU5402 and (iii) WT embryos treated with 10uM DEAB. The genes assayed include a tox3, b sema3fb, c mpz, d gas6, e celf2, f nr2f2, g col15a1b and h gbx1. krox20 (red), which is expressed in r3 and r5, was used to position the expression domain of some genes as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging

Expression Data

Genes:	celf2 col15a1b egr2b gas6 gbx1 mpz nr2f2 sema3fb tox3
Fish:	WT
Conditions:	chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde chemical treatment by environment: SU5402
Anatomical Terms:	hindbrain rhombomere 1 rhombomere 2 rhombomere 3 rhombomere 5 rhombomere 6
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde chemical treatment by environment: SU5402
Observed In:	hindbrain rhombomere 5 rhombomere 6
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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