FIGURE

Fig. 7

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ZDB-FIG-180711-31

Publication

Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development

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Fig. 7

RA signaling is unaffected by the loss of PG1 hox genes.

The expression of aldh1a2 at 70% epiboly is unchanged in paralog group 1 mutants (B, C, D) as compared to wildtype (A). The loss of hoxb1b causes a downregulation of cyp26c1 (hoxb1b^−/−: 16/17 embryos, hoxb1a^−/−;hoxb1b^−/−: 7/7 embryos)(G, H) and cyp26b1 (hoxb1b^−/−: 20/20 embryos, hoxb1a^−/−;hoxb1b^−/−: 8/8 embryos)(K, L) in r4. hoxb1a^−/− mutants show no reduction in the expression of cyp26c1 (F) or cyp26b1 (J) in r4. The expression of RA dependent genes, hoxd4a (M-P) and meis3 (Q-T) are unaffected by the loss of paralog group 1 hox genes. All embryos have been genotyped for hoxb1a^sa1191 and hoxb1b^ua1006. All images are dorsal views, (A-D) anterior to the top, 70% epiboly, (E-T) anterior to the left, 4 somites.

Expression Data

Genes:	aldh1a2 cyp26b1 cyp26c1 hoxd4a meis3
Fish:	AB hoxb1a^{sa1191/sa1191} hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Anatomical Terms:	hindbrain neural plate margin presumptive rhombomere 2 presumptive rhombomere 3 presumptive rhombomere 4 somite
Stage Range:	75%-epiboly to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1a^{sa1191/sa1191} hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Observed In:	presumptive rhombomere 3 presumptive rhombomere 4 rhombomere 2 rhombomere 3 rhombomere 3 anterior/posterior compartment boundary rhombomere 4 rhombomere 4 anterior/posterior compartment boundary
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.