FIGURE

Fig. S2

ID

ZDB-FIG-180711-37

Publication

Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development

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Fig. S2

Alterations to rhombomere size in paralog group 1 hox mutants.

Wildtype embryos show the normal expression patterns for epha4a in r3/5 (A), krox20 in red in r3/5 and val in purple in r5/6 (E), and hoxa3a (I) and hoxb3a (M) in r5/6. Expression of all markers are unaffected in hoxb1a^−/− mutants (B,F,J,N). Hoxb1b^−/− mutants have an increase in the A-P extent of r3 as observed in epha4a (C) and krox20 (G) expression. The A-P extent of r4 is reduced in hoxb1b^−/− mutants as seen in the space between r3/5 staining of epha4a (C) and krox20 (G). The A-P extent of r5/6 is also slightly reduced in hoxb1b^−/− mutants as seen by val (G), hoxa3a (K) and hoxb3a (O) expression. Hoxb1a^−/−;hoxb1b^−/− mutants have a similar expansion or r3 and reduction in the A-P extent of r4 as evidenced by epha4a (D) and krox20 (H) expression. The A-P extent of r5/6 is also reduced in hoxb1a^−/−;hoxb1b^−/− mutants as seen by val (H), and hoxb3a (P) expression. Hoxb1a^−/−;hoxb1b^−/− mutants also have a decrease in the expression of hoxa3a in r6 (L). All embryos have been genotyped for hoxb1a^sa1191 and hoxb1b^ua1006. All embryos are dorsal views, anterior to the left, (A-D) 22hpf, (E-P) 18hpf. hpf, hours post fertilization.

Expression Data

Genes:	egr2b epha4a hoxa3a hoxb3a mafba
Fish:	AB hoxb1a^{sa1191/sa1191} hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Anatomical Terms:	rhombomere 3 rhombomere 5 rhombomere 6
Stage Range:	14-19 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Observed In:	rhombomere 3 rhombomere 5 rhombomere 6
Stage Range:	14-19 somites to 26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.