FIGURE

Fig. 6

ID

ZDB-FIG-180711-30

Publication

Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development

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Fig. 6

PG1 hox genes regulate vhnf1 in a retinoic acid independent mechanism.

In order to determine if the decrease in vhnf1 expression observed in hoxb1a^−/−hoxb1b^−/− mutants is due to defects in RA, embryos were treated with 5 nM RA(A,B) or DMSO (C,D) and then vhnf1 expression was analyzed. Wildtype embryos treated with 5 nM RA (A) showed an increase in vhnf1 expression as compared to those treated with DMSO (C). In hoxb1a^−/−;hoxb1b^−/− mutants, there is an increase in expression of vhnf1 in embryos treated with RA (3/3 embryos)(B), as compared to controls (D), however vhnf1 expression has not recovered to wildtype levels (A). All embryos have been genotyped for hoxb1a^sa1191 and hoxb1b^ua1006. All images are dorsal views, anterior to the left, 4 somites.

Expression Data

Gene:	hnf1ba
Fish:	AB hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Condition:	chemical treatment by environment: retinoic acid
Anatomical Term:	hindbrain neural plate
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Condition:	chemical treatment by environment: retinoic acid
Observed In:	hindbrain neural plate
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.