FIGURE

Fig. 4

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ZDB-FIG-161205-4
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Cayuso et al., 2016 - EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality
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Fig. 4

Complementary EphrinB1 and EphB3b Expression Controls Hepatoblast Positioning and Cell-Shape Changes in Liver Bud Formation

(A-F‴) At 22 hpf, EphrinB1 and EphB3b expression largely overlaps in future hepatoblasts and the LPM (A-A‴). Complementary expression of both factors coincides with the start of liver budding: EphrinB1 in hepatoblasts and EphB3b in the gut and LPM (B–B‴). At 32 hpf, hepatoblasts are located more posteriorly and medially compared with controls in MO-ephrinb1 (C-D‴), in MO-ephb3b (E-E‴), and upon conditional UAS:ephrinb1EC expression (F-F‴). (A-F′) ventral views of confocal projections, anterior to the top; (A″–F″) transverse sections of the foregut, as indicated by the dashed line in (A′-F′), and matching schematics (A‴-F‴); yellow arrowheads specify the midline and white brackets the length of the Prox1 domain.

(G–I) Cell shapes were determined with EphrinB1-staining at 32 hpf (see Figure 1F). Quantification of hepatoblast shape in control, MO-ephrinb1, and MO-ephb3b embryos: (G) L/W distribution for one representative bud; (H) proportion of elongated cells per bud; SEs are shown; and (I) orientation of elongated hepatoblasts with respect to the anteroposterior axis. p < 0.05.

See also Figure S2.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 26+ somites to Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 39, Cayuso, J., Dzementsei, A., Fischer, J.C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G.J., Ober, E.A., EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality, 316-328, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell