FIGURE

Fig. 5

ID

ZDB-FIG-161205-5

Publication

Cayuso et al., 2016 - EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality

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Fig. 5

EphB3b Controls LPM Polarity and Asymmetric Movement

(A-E′) EphrinB1 and EphB3b regulate LPM polarity in the foregut region. α-ZO-1 staining in MO-ephb3b embryos reveals that junctions in the LPM form similar to controls at 22 hpf (A-B′); while at 32 hpf, ZO-1 is mislocalized (arrowheads) in MO-ephrinb1 and MO-ephb3b embryos (C-E′). Yellow arrowheads specify the midline and reveal impaired gut looping in MO-ephrinb1 and MO-ephb3b (C-E).

(F-G′) casanova mutants exhibit ZO-1 localization defects in the LPM (arrowheads) at 32 hpf.

(A-G′) Transverse sections at liver level, left side to the right; dashed lines delineate the endoderm (green) and LPM (yellow); (A′-G′) are magnifications of the areas indicated by a box in (A-G).

Expression Data

Genes:	efnb1 prox1a
Antibody:	Ab1-tjp1
Fish:	WT sox32^ta56/ta56 WT + MO1-ephb3b + MO2-ephb3b WT + MO2-efnb1 + MO3-efnb1 zf106Tg zf106Tg + MO1-ephb3b + MO2-ephb3b zf106Tg + MO2-efnb1 + MO3-efnb1
Knockdown Reagents:	MO1-ephb3b MO2-efnb1 MO2-ephb3b MO3-efnb1
Anatomical Terms:	hepatoblast intestinal rod lateral plate mesoderm lateral plate mesoderm apical junction complex mesoderm apical junction complex
Stage Range:	26+ somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox32^ta56/ta56 WT + MO1-ephb3b + MO2-ephb3b WT + MO2-efnb1 + MO3-efnb1
Knockdown Reagents:	MO1-ephb3b MO2-efnb1 MO2-ephb3b MO3-efnb1
Observed In:	embryonic digestive tract development intestinal bulb primordium lateral plate mesoderm establishment of epithelial cell apical/basal polarity mesoderm apical junction complex
Stage Range:	26+ somites to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 39, Cayuso, J., Dzementsei, A., Fischer, J.C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G.J., Ober, E.A., EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality, 316-328, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell