FIGURE

Fig. S4

ID

ZDB-FIG-160715-23

Publication

Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

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Fig. S4

The decreased products of Pol III in rpc9^-/- mutants, the expression pattern of polr3h and polr3k and the evaluation of their morpholinos. (A) qRT-PCR revealed that, compared to rpc9^+/+embryos, the level of pre-tRNA^Ile, pre-tRNA^Leu, 5S rRNA and 7SL RNA was significantly decreased in rpc9^-/-embryos at 5 dpf (mean±SD, *P < 0.05, ***P < 0.001). (B) The expression profiles of polr3h and polr3k. (C) The EGFP signal in zebrafish embryos injected with pEGFPN1-polr3h, or embryos co-injected with pEGFPN1-polr3h and polr3h MO (upper panel); The EGFP signal in zebrafish embryos injected with pEGFPN1-polr3k, or embryos co-injected with pEGFPN1-polr3k and polr3k MO (lower panel); (D) The expression pattern of flk1 at 36 hpf and foxn1 at 5 dpf in control embryos, polr3h and polr3k morphants.

Expression Data

Genes:	polr3h polr3k
Fish:	AB
Anatomical Term:	hematopoietic system posterior region
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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