FIGURE

Fig. 5

ID

ZDB-FIG-160715-18

Publication

Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

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Fig. 5

Knockdown of Pol III components polr3h and polr3k leads to HSPC defects. (A) qRT-PCR result shows that, compared with control embryos, products of Pol III (pre-tRNA^Ile, pre-tRNA^Leu, 5S rRNA and 7SL RNA) are all significantly decreased in both polr3h and polr3k morphants (5dpf). 18S rRNA was used as internal control. (B) WISH result showing that HSPC marker (cmyb) and differentiated hematopoietic cell lineage markers (gata1, pu.1 and rag1) are all decreased in polr3h and polr3k morphants at 5dpf. (C) qRT-PCR result reveals that cmyb, gata1 and pu.1 are significantly decreased in polr3h and polr3k morphants at 5dpf. β-actin was used as internal control. Note that arrowheads in B mark the CHT or the thymus. Values are mean±s.d. **P<0.01, ***P<0.001, Student′s t-test.

Expression Data

Genes:	gata1a myb rag1 spi1b
Fish:	AB AB + MO1-polr3h AB + MO1-polr3k
Knockdown Reagents:	MO1-polr3h MO1-polr3k
Anatomical Terms:	hematopoietic multipotent progenitor cell hematopoietic system posterior region thymus whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-polr3h AB + MO1-polr3k
Knockdown Reagents:	MO1-polr3h MO1-polr3k
Observed In:	hematopoietic multipotent progenitor cell hematopoietic system posterior region thymus thymus T cell whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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