Fig. S2

Hematopoietic cells are affected at 5 dpf but not at early stage in rpc9^-/- embryos. (A) WISH result demonstrated the expression pattern of pu.1, lyz and mfap4 in the thymus of rpc9^-/- or rpc9^+/+ embryos at 4.5 dpf. Red arrowheads mark the thymus. (B) WISH result demonstrated that, compared to that of rpc9^+/+ embryos, gata1, l-plastin and pu.1 were severely decreased in the CHT region of rpc9^-/- embryos at 5 dpf. Arrowheads mark the CHT. (C) qRT-PCR result revealed that, compared with rpc9^+/+ embryos, gata1, l-plastin and pu.1 were all significantly decreased in rpc9^-/- embryos, at 5dpf. β-actin was used as internal control (mean±SD, ***P0.001). (D) WISH result demonstrated that, compared to that of rpc9^+/+ embryos, scl, gata1 and pu.1 were not obviously altered in rpc9^-/- embryos at 10 somite stage. Arrowheads mark the ALM(anterior lateral mesoderm) or PLM (posterior lateral mesoderm)region of zebrafish embryos. (E) WISH result demonstrated that, compared to the rpc9^+/+ embryos, scl, gata1 and pu.1 in rpc9^-/- embryos were not obviously altered at 25 and 36 hpf. Arrowheads mark the AGM or the CHT region.