Fig. S6

The absence of rag1 and excessive apoptosis in rpc9^-/- embryos can be rescued by knockdown of P53. (A) qRT-PCR result revealed that p53 was significantly increased in rpc9^-/- embryos at 3 and 4 dpf. β-actin was used as internal control (mean±SD, *P < 0.05, **P < 0.01) . (B) WISH result demonstrated the decrease of rag1 in the thymus of rpc9^-/- embryos at 5 dpf could be rescued by p53-deficiency. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. Arrowheads mark the thymus. (C) TUNEL assay demonstrated apoptosis in the CHT of rpc9^+/+ and rpc9^-/- embryos that injection with control or p53 MO at 4 dpf. (D) Statistical data of (C) revealed that apoptosis signals in rpc9^-/- embryos could be significantly repressed by injection of p53 MO at 4 dpf (mean±SEM, ***P < 0.001). (E) pH3 assay demonstrated proliferation in the CHT of rpc9^+/+ and rpc9^-/- embryos that injection with control or p53 MO at 4 dpf. (E) Statistical data of (F) revealed that proliferation signals were not significantly altered between rpc9^-/- embryos that injected with control or p53 MO at 4 dpf (mean±SEM, ns, no significant). (G) WISH showed no obvious alteration of expression of arterial markers dlc and dll4 in rpc9-/- embryos.