FIGURE

Fig. 6

ID

ZDB-FIG-160715-19

Publication

Wei et al., 2016 - RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

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Fig. 6

P53 mediates the regulation of Rpc9 in HSPC survival. (A) WISH result reveals that p53 is specifically increased in the CHT region of rpc9^-/- embryos at 3 and 4dpf. (B) Western blot shows that, compared with rpc9^+/+ embryos, the protein level of P53 was greatly increased in rpc9^-/- embryos at 4dpf. (C) The quantitative result of the western blot in B. (D) The absence of rag1 in the thymus of rpc9^-/- embryos, at 5dpf, can be rescued by loss of p53. (E) The absence of cmyb in the CHT region of rpc9^-/- embryos at 5dpf can be rescued by p53 deficiency. (F) qRT-PCR result reveals that the decrease of cmyb in the CHT region of rpc9^-/- embryos at 5dpf can be rescued by loss of p53. Note that arrowheads in A,E mark the CHT, whereas arrowheads in D mark the thymus. Values are mean±s.e.m. ***P<0.001, Student′s t-test.

Expression Data

Genes:	myb rag1 tp53
Antibody:	Ab13-tp53
Fish:	AB crcp^hkz8/hkz8 tp53^zdf1/zdf1 crcp^hkz8/hkz8; tp53^zdf1/zdf1
Anatomical Terms:	hematopoietic multipotent progenitor cell hematopoietic system posterior region thymus thymus T cell whole organism
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	crcp^hkz8/hkz8 crcp^hkz8/hkz8; tp53^zdf1/zdf1
Observed In:	hematopoietic multipotent progenitor cell hematopoietic system posterior region thymus whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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