FIGURE

Fig. S5

ID

ZDB-FIG-121030-26

Publication

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation

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Fig. S5

Mef2ca and Mef2cb dual loss of function abolishes myocardial differentiation.
In situ mRNA hybridisation for myl7 (A), vmhc (B), bmp4 (C), smyd1b (D) and gata4,5,6 (E) in hearts of zebrafish embryos shown in a dorsal view, anterior to top (A-C and E) or in lateral view, anterior to left (D). A,B. Myl7 and vmhc expression in hearts (arrowheads) is lost after dual loss of function of Mef2ca and Mef2cb by various combinations of MOs and mutants. C. Bmp4 is dramatically reduced from heart of 22 ss mef2d/c morphants. D. Smyd1b mRNA is abolished from hearts (arrowheads) of 22 ss mef2d/c morphants. Expression in somites is slightly downregulated (arrow). E. Gata4, gata5 and gata6 appear unchanged in 22 ss mef2d/c morphants. Scale = 100 μm.

Expression Data

Genes:	bmp4 gata4 gata5 gata6 myh7 myl7 smyd1b
Fish:	WT WT + MO1-mef2d mef2ca^tn213/tn213 + MO2-mef2cb WT + MO1-mef2ca + MO2-mef2cb WT + MO3-mef2d,mef2ca
Knockdown Reagents:	MO1-mef2ca MO1-mef2d MO2-mef2cb MO3-mef2d,mef2ca
Anatomical Terms:	anterior lateral plate mesoderm heart primordium heart rudiment lateral plate mesoderm somite
Stage Range:	10-13 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mef2ca^tn213/tn213 + MO2-mef2cb WT + MO1-mef2ca + MO2-mef2cb WT + MO3-mef2d,mef2ca
Knockdown Reagents:	MO1-mef2ca MO2-mef2cb MO3-mef2d,mef2ca
Observed In:	cardiac muscle cell cardiac muscle cell differentiation heart heart formation heart rudiment
Stage Range:	14-19 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.