FIGURE

Fig. 5

ID

ZDB-FIG-121030-37

Publication

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation

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Fig. 5

Cardiomyocytes of mef2ca;mef2cb dual loss of function are specified but developmentally arrested. In situ mRNA hybridisation for indicated genes (wholemounts in dorsal view, anterior to top, except J, ventral view). A–C. ALPM expression (arrowheads) of nkx2.5 (A), hand2 (B) and gata4 (C) at 12 ss is unaffected by lack of Mef2ca and Mef2cb. Notochord and rhombomeres 3 and 5 are marked by ntl (blue) and egr2b (red), respectively. D,E.Tbx20 and gata6 mRNAs are present but disorganised in the heart region of mef2d/c morphants and mef2ca^b1086;mef2cb^fh288 mutant embryos (lacking myl7 expression in red, E, right panel) compared to the typical ring-shape in control and sibling embryos. F. During heart cone stage (22 ss), nkx2.5 mRNA is abolished. G. hand2 mRNA is enhanced in a sheet of cells spanning the cardiac region but not elsewhere. Pharyngeal pouch expression is unchanged (white arrowheads). H, Whereas myocardial cells are undifferentiated in mef2d/c morphants and mef2ca^b1086;mef2cb^fh288 mutants (myl7, red), the endocardium is expanded and cdh5 is up-regulated. I. Endocardium (cdh5, arrow) lines the myocardium (myl7, red) in the normal looped heart of a sibling embryo. In mef2ca;mef2cb mutant embryos, little endocardial makrer is co-localised with the residual myocardium (arrows). Extra cdh5-expressing tissue is detected in the cardiac region (arrowheads). Scale=100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Genes:	cdh5 gata4 gata6 hand2 myl7 nkx2.5 tbx20
Fish:	WT mef2ca^b1086/b1086 + MO2-mef2cb + MO3-mef2cb WT + MO3-mef2d,mef2ca mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagents:	MO2-mef2cb MO3-mef2cb MO3-mef2d,mef2ca
Anatomical Terms:	anterior lateral plate mesoderm endocardium heart rudiment myocardium pharyngeal pouch
Stage Range:	10-13 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-mef2d,mef2ca mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagent:	MO3-mef2d,mef2ca
Observed In:	endothelial cell heart heart formation heart rudiment cardiac muscle cell
Stage Range:	20-25 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.