ZFIN Image: Hinits et al., 2012, Fig. S5

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Figure Caption

Fig. S5 Mef2ca and Mef2cb dual loss of function abolishes myocardial differentiation.
In situ mRNA hybridisation for myl7 (A), vmhc (B), bmp4 (C), smyd1b (D) and gata4,5,6 (E) in hearts of zebrafish embryos shown in a dorsal view, anterior to top (A-C and E) or in lateral view, anterior to left (D). A,B. Myl7 and vmhc expression in hearts (arrowheads) is lost after dual loss of function of Mef2ca and Mef2cb by various combinations of MOs and mutants. C. Bmp4 is dramatically reduced from heart of 22 ss mef2d/c morphants. D. Smyd1b mRNA is abolished from hearts (arrowheads) of 22 ss mef2d/c morphants. Expression in somites is slightly downregulated (arrow). E. Gata4, gata5 and gata6 appear unchanged in 22 ss mef2d/c morphants. Scale = 100 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.