FIGURE

Fig. S6

ID

ZDB-FIG-121030-27

Publication

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation

Other Figures

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Fig. S6

Mef2ca and Mef2cb dual loss of function abolishes myocardial differentiation.
A,B. In situ mRNA hybridisation for myl7 in hearts of 24 hpf mef2ca^b1086;mef2cb^fh288 mutant embryos and their siblings (A) or control and mef2cb^+/fh288 incross embryos non-injected or injected with mef2ca MO, shown in a dorsal view, anterior to top. Double mutants have almost no myl7 expression albeit few cells in the cardiac region (arrows, A). Mef2cb^fh288 mutant embryos injected with mef2ca MO have a thinner than normal heart tube. All other control or embryos with only one Mef2 depleted have a normal heart. C. Bright field images of mef2cbfh288 mutant embryos injected with mef2ca MO showing cardiac edema, compared with mef2ca MO injected siblings with normal heart and no edema. Scale = 100 μm.

Expression Data

Gene:	myl7
Fish:	WT WT + MO1-mef2ca mef2cb^fh288/+ mef2cb^fh288/fh288 + MO1-mef2ca mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagent:	MO1-mef2ca
Anatomical Terms:	cardiac muscle cell heart tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mef2ca mef2cb^fh288/+ mef2cb^fh288/fh288 + MO1-mef2ca mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagent:	MO1-mef2ca
Observed In:	heart tube pericardium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.