ZFIN Image: van der Velden et al., 2011, Fig. S5

Image

Figure Caption

Fig. S5

Zebrafish TOR (zTOR) signaling is not grossly deregulated in lkb1-mutant intestine. Transverse sections of WT and lkb1-mutant intestines at indicated days of development were stained with an antibody against phospho-S6 ribosomal protein (RS6, Ser240/244) (A–E) and phospho-4EBP1 (Thr-37/46) (F–J). Strong phospho-RS6 staining is detected in WT and lkb1-mutant intestines at 5 dpf (A and B). Food-deprived WT larvae show moderate staining at 7 and 11 dpf (C and E). Staining in lkb1 mutants (B and D) is not overtly different from staining in age-matched WT larvae (A and C). Moderate phospho-4EBP1 stating is detected in WT and lkb1-mutant intestines at 5 dpf. (F and G). Food-deprived WT larvae show decreased staining at 7 and 11 dpf (H and J). Staining in lkb1 mutants is similar to that in age-matched WT larvae (G and I). Red arrows indicate expression in the pancreas.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA