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Fig. 4
The zebrafish TOR (zTOR) pathway activity in lkb1 mutants resembles that in starved WT larvae. (A) Western blot analysis with antibodies against phospho-AMPK and phospho-ACC of total protein lysates from embryos at the indicated days and genotypes. AMPK and ACC are not phosphorylated in WT or lkb1-mutant larvae at 5 or 7 dpf. Prolonged fasting induces phosphorylation of AMPK and ACC in 11-dpf WT larvae. Elevated expression of AMPK in 7-dpf lkb1-mutant larvae is detected. (B) Western blot analysis using anti–phospho-p70S6K, anti-p70S6K, anti–phospho-4EBP1, anti-4EBP1, anti–phospho-RS6, and anti-RS6 antibodies of total protein lysates from embryos at the indicated days and genotypes. (C) Sections of livers obtained from WT and lkb1-mutant embryos at the indicated days stained with anti–phospho-RS6 and anti–phospho-4EBP1.